WB
Western blot analysis of various lysates, using [KD Validated] METTL14 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.IHC
Immunohistochemistry analysis of METTL14 in paraffin-embedded human colon carcinoma using [KO Validated] METTL14 Rabbit mAb at dilution of 1:2000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.IP
Immunoprecipitation analysis of 300ug extracts of Raji cells using 3ug METTL14 Rabbit mAb. Western blot was performed from the immunoprecipitate using METTL14 Rabbit mAb at a dilition of 1:500.| Product Name | [KD Validated] METTL14 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:1000 IHC:1:1000-1:5000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | METTL14 |
|---|---|
| Gene Synonyms | hMETTL14 |
| Gene Full Name | methyltransferase 14, N6-adenosine-methyltransferase non-catalytic subunit |
| Gene Summary | Enables mRNA binding activity. Contributes to mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity. Involved in mRNA metabolic process; negative regulation of hematopoietic progenitor cell differentiation; and positive regulation of translation. Located in nucleoplasm. Part of RNA N6-methyladenosine methyltransferase complex. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | hMETTL14 [KD Validated] METTL14 |
| Molecular Weight(MW) | Calculated MW 52kDa(Observed MW 65KDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of various lysates, using [KD Validated] METTL14 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
IHC
Immunohistochemistry analysis of METTL14 in paraffin-embedded human colon carcinoma using [KO Validated] METTL14 Rabbit mAb at dilution of 1:2000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
IP
Immunoprecipitation analysis of 300ug extracts of Raji cells using 3ug METTL14 Rabbit mAb. Western blot was performed from the immunoprecipitate using METTL14 Rabbit mAb at a dilition of 1:500.| Application Notes | WB:1:500-1:1000 IHC:1:1000-1:5000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
