WB
Mouse heart lysates were subjected to SDS PAGE followed by western blot with MYL7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffinembedded mouse heart tissue slide using MYL7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of(4%PFA) fixed mouse heart tissue using MYL7 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-MYL7 (IP:4ug; Detection:1:700) with mouse heart tissue lysate 4000ug.| Product Name | MYL7 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | MYL7 fusion protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCIPWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0mg oftotal protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 650ug/ml |
| Purification | Affinity purified |
| Gene Symbol | MYL7 |
|---|---|
| Gene Synonyms | MYL2A MYLC2A |
| Gene Full Name | myosin light chain 7 |
| Gene Summary | Predicted to enable calcium ion binding activity. Located in A band. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | MLC2a MLC-2a MYL2A MYL7/MLC2a MYLC2A |
| Molecular Weight(MW) | 19kDa |
WB
Mouse heart lysates were subjected to SDS PAGE followed by western blot with MYL7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffinembedded mouse heart tissue slide using MYL7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of(4%PFA) fixed mouse heart tissue using MYL7 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-MYL7 (IP:4ug; Detection:1:700) with mouse heart tissue lysate 4000ug.| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0mg oftotal protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year aftershipment. |
| Storage Buffer | PBS with 0.02%sodium azide and 50%glycerol pH 7.3 |
