WB
mouse testis tissue were subjected to SDS PAGE followed by western blot with BubR1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using BubR1 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using BubR1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | BubR1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | BubR1 fusion protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG2 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:200-1:1000 ICC:1:20-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 300ug/ml |
| Purification | Affinity purified |
| Gene Symbol | BUB1B |
|---|---|
| Gene Synonyms | MVA1 SSK1 BUBR1 Bub1A MAD3L hBUBR1 BUB1beta |
| Gene Full Name | BUB1 mitotic checkpoint serine/threonine kinase B |
| Gene Summary | This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008] |
| Alternative Names | BubR1 Bub1A BUB1beta EC:2.7.11.1 hBUBR1 |
| Molecular Weight(MW) | 120-130kDa |
WB
mouse testis tissue were subjected to SDS PAGE followed by western blot with BubR1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using BubR1 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using BubR1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:1000 IHC:1:200-1:1000 ICC:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
