WB
Western blot analysis of Cathepsin S on different lysates with Rabbit anti-Cathepsin S antibody at 1/1,000 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Lane 4: A431 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 25/35/40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of SiHa cells labeling Cathepsin S with Rabbit anti-Cathepsin S antibody at 1/200 dilution.. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin S antibodyat 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.| Product Name | Cathepsin S Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Cathepsin S aa 101-331 / 331. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTSS |
|---|---|
| Gene Full Name | cathepsin S |
| Gene Summary | The preproprotein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that participates in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The mature protein cleaves the invariant chain of MHC class II molecules in endolysosomal compartments and enables the formation of antigen-MHC class II complexes and the proper display of extracellular antigenic peptides by MHC-II. The mature protein also functions as an elastase over a broad pH range. When secreted from cells, this protein can remodel components of the extracellular matrix such as elastin, collagen, and fibronectin. This gene is implicated in the pathology of many inflammatory and autoimmune diseases and, given its elastase activity, plays a significant role in some pulmonary diseases. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2020] |
| Alternative Names | Cathepsin S antibody CathepsinS antibody CATS_HUMAN antibody Ctss antibody |
| Molecular Weight(MW) | 37kDa（25/35/40 kDa） |
| Cellular Localization | Lysosome, Secreted, Cytoplasmic vesicle, phagosome. |
WB
Western blot analysis of Cathepsin S on different lysates with Rabbit anti-Cathepsin S antibody at 1/1,000 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Lane 4: A431 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 25/35/40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of SiHa cells labeling Cathepsin S with Rabbit anti-Cathepsin S antibody at 1/200 dilution.. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin S antibodyat 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
