WB
Western blot analysis using ASS1 antibody against COS7(1),T24(2),MCF-7(3),C2C12(4) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (
OM790003), and block it with TBST (
OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (
OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (
OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (
OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (
OM750016) again, 5 minutes each time. Use ECL (
OM625701) for luminescence.staining time: 60S.
ICC/IF
Immunofluorescence analysis of U251 cells usingASS1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (
OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (
OM750003). Block the tissue with 10% non-immune goat serum(
OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (
OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(
OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.