WB
Variouslysates were subjected to SDS PAGE followed by western blotwith SGLT2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffinembedded rat kidney tissue slide using SGLT2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).