WB
Western blot analysis of SGLT2/SLC5A2 using anti-SGLT2/SLC5A2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SGLT2/SLC5A2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of SGLT2/SLC5A2 using anti-SGLT2/SLC5A2 antibody. SGLT2/SLC5A2 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SGLT2/SLC5A2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of SGLT2/SLC5A2 using anti-SGLT2/SLC5A2 antibody. SGLT2/SLC5A2 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SGLT2/SLC5A2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of SGLT2/SLC5A2 using anti-SGLT2/SLC5A2 antibody. SGLT2/SLC5A2 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SGLT2/SLC5A2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of HepG2 cells using anti- SLC5A2 antibody. Overlay histogram showing HepG2 cells stained with Anti-SGLT2/SLC5A2 Antibody (Blue line).. And then incubated with rabbit anti-SLC5A2 Antibody (1:100) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to SGLT2/SLC5A2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human SGLT2/SLC5A2 recombinant protein (Position: A15-N656). Human SGLT2/SLC5A2 shares 91.3% and 91.4% amino acid (aa) sequence identity with mouse and rat SGLT2/SLC5A2, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC5A2 |
|---|---|
| Gene Synonyms | SGLT2 |
| Gene Full Name | solute carrier family 5 member 2 |
| Gene Summary | This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene. [provided by RefSeq, Feb 2015] |
| Alternative Names | Na(+)/glucose cotransporter 2 SGLT2 SLC5A2 Sodium/glucose cotransporter 2 |
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Cell membrane,Membrane. |
| SwissProt ID | P31639 |
|---|
WB
Western blot analysis of SGLT2/SLC5A2 using anti-SGLT2/SLC5A2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SGLT2/SLC5A2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of SGLT2/SLC5A2 using anti-SGLT2/SLC5A2 antibody. SGLT2/SLC5A2 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SGLT2/SLC5A2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of SGLT2/SLC5A2 using anti-SGLT2/SLC5A2 antibody. SGLT2/SLC5A2 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SGLT2/SLC5A2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of SGLT2/SLC5A2 using anti-SGLT2/SLC5A2 antibody. SGLT2/SLC5A2 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SGLT2/SLC5A2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of HepG2 cells using anti- SLC5A2 antibody. Overlay histogram showing HepG2 cells stained with Anti-SGLT2/SLC5A2 Antibody (Blue line).. And then incubated with rabbit anti-SLC5A2 Antibody (1:100) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
