WB
Western blot analysis of anti- ABP1/AOC1 antibody. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole cell lysates, Lane 2: HK-2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. Use rabbit anti- ABP1/AOC1 1:1000, probed with a goat antirabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for ABP1/AOC1 at approximately 85KD. The expected band size for ABP1/AOC1 is at 85KD.IHC
IHC analysis using anti-ABP1/AOC1 antibody.detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex(SABC) with DAB as the chromogen.ICC/IF
ICC analysis using ABP1/AOC1 antibody. was detected in immersion fixed T47D cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)and counterstained with DAPI (blue).FCM
Flow Cytometry analysis of THP-1 cells using anti-ABP1/AOC1 antibody. Overlay histogram showing THP-1 cells stained with A08386-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ABP1/AOC1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-ABP1/AOC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ABP1/AOC1 recombinant protein (Position: S140-R180). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCMICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FCM:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Symbol | AOC1 |
|---|---|
| Gene Synonyms | ABP DAO KAO ABP1 DAO1 KDAO |
| Gene Full Name | amine oxidase copper containing 1 |
| Gene Summary | This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] |
| Alternative Names | ABP ABP1 Amiloride binding protein AOC1 DAO DAO1 Diamine oxidase Histaminase KAO Kidney amine oxidase |
| Molecular Weight(MW) | 85kDa |
| Cellular Localization | Cell membrane,Membrane,Secreted |
WB
Western blot analysis of anti- ABP1/AOC1 antibody. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole cell lysates, Lane 2: HK-2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. Use rabbit anti- ABP1/AOC1 1:1000, probed with a goat antirabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for ABP1/AOC1 at approximately 85KD. The expected band size for ABP1/AOC1 is at 85KD.
IHC
IHC analysis using anti-ABP1/AOC1 antibody.detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex(SABC) with DAB as the chromogen.
ICC/IF
ICC analysis using ABP1/AOC1 antibody. was detected in immersion fixed T47D cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)and counterstained with DAPI (blue).
FCM
Flow Cytometry analysis of THP-1 cells using anti-ABP1/AOC1 antibody. Overlay histogram showing THP-1 cells stained with A08386-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ABP1/AOC1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FCM:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
