WB
Western blot analysis of SAV1 with Rabbit anti-SAV1 antibody at 1/2,000 dilution at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: MDA-MB-231 cell lysate (20 µg/Lane) Lane 6: SW480 cell lysate (20 µg/Lane) Lane 7: BxPC-3 cell lysate (20 µg/Lane) Lane 8: 786-0 cell lysate (negative) (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: NIH/3T3 cell lysate (20 µg/Lane) Lane 11: PC-12 cell lysate (20 µg/Lane) Lane 12: Mouse testis tissue lysate (40 µg/Lane) Lane 13: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minute4; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of HCT 116 cells labeling SAV1. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | SAV1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SAV1 aa 1-383 / 383. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:2000 IHC:1:200-1:1000 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SAV1 |
|---|---|
| Gene Synonyms | SAV WW45 WWP4 |
| Gene Full Name | salvador family WW domain containing protein 1 |
| Gene Summary | WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein with two WW domains, a SARAH domain, and a coiled-coil region and is ubiquitously expressed in adult tissues. This protein binds to MST1 (mammalian sterile 20-like kinase 1) and promotes MST1-induced apoptosis. It has also been shown to bind to HAX1 (hematopoietic cell-specific protein 1 (HS1)-associated protein X-1) and to attenuate the anti-apoptotic effects of HAX1. Studies in human and mouse suggest this gene acts as a tumor suppressor. [provided by RefSeq, Aug 2012] |
| Alternative Names | 1700040G09Rik antibody 45 kDa WW domain protein antibody hWW 45 antibody hWW45 antibody Protein salvador homolog 1 antibody salvador family WW domain containing protein 1 antibody salvador homolog 1 (Drosophila) antibody Salvador homolog 1 antibody Salvador Drosophila homolog of antibody SAV 1 antibody 1700040G09Rik antibody 45 kDa WW domain protein antibody hWW 45 antibody hWW45 antibody Protein salvador homolog 1 antibody salvador family WW domain containing protein 1 antibody salvador homolog 1 (Drosophila) antibody Salvador homolog 1 antibody Salvador Drosophila homolog of antibody SAV 1 antibody SAV antibody SAV1 antibody SAV1_HUMAN antibody WW 45 antibody WW domain containing antibody WW domain-containing adaptor 45 antibody WW domain-containing protein 45-KD antibody WW45 antibody WW45 protein antibody WWP 4 antibody WWP4 antibody |
| Molecular Weight(MW) | 45kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
WB
Western blot analysis of SAV1 with Rabbit anti-SAV1 antibody at 1/2,000 dilution at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: MDA-MB-231 cell lysate (20 µg/Lane) Lane 6: SW480 cell lysate (20 µg/Lane) Lane 7: BxPC-3 cell lysate (20 µg/Lane) Lane 8: 786-0 cell lysate (negative) (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: NIH/3T3 cell lysate (20 µg/Lane) Lane 11: PC-12 cell lysate (20 µg/Lane) Lane 12: Mouse testis tissue lysate (40 µg/Lane) Lane 13: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minute4; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of HCT 116 cells labeling SAV1. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:200-1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
