WB
Variouslysates were subjected to SDS PAGE followed by western blot with Ajuba antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffinembedded mouse skin tissue slide using Ajuba antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).| Product Name | Ajuba Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Ajuba fusion protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:6000 IHC:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AJUBA |
|---|---|
| Gene Synonyms | JUB |
| Gene Full Name | ajuba LIM protein |
| Gene Summary | Enables alpha-catenin binding activity and transcription corepressor activity. Involved in several processes, including negative regulation of hippo signaling; positive regulation of gene silencing by miRNA; and regulation of cellular response to hypoxia. Acts upstream of or within gene silencing by miRNA and positive regulation of protein-containing complex assembly. Located in several cellular components, including Golgi apparatus; P-body; and nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | JUB LIM domain-containing protein ajuba Protein ajuba |
| Molecular Weight(MW) | 57kDa |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Cytoskeleton,Membrane,Nucleus |
WB
Variouslysates were subjected to SDS PAGE followed by western blot with Ajuba antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffinembedded mouse skin tissue slide using Ajuba antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).| Application Notes | WB:1:1000-1:6000 IHC:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
