WB
Various lysates were subjected to SDS PAGE followed by western blot with TIGIT antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TIGIT antibody at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | TIGIT Recombinant antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Fusion Protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC:1:125-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TIGIT |
|---|---|
| Gene Synonyms | VSIG9 VSTM3 WUCAM |
| Gene Full Name | T cell immunoreceptor with Ig and ITIM domains |
| Gene Summary | This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009] |
| Alternative Names | VSTM3 VSIG9 V-set and transmembrane domain-containing protein 3 V-set and immunoglobulin domain-containing protein 9 T-cell immunoreceptor with Ig and ITIM domains |
| Molecular Weight(MW) | 26kDa(Observed MW: 44kDa) |
| Cellular Localization | Cell membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with TIGIT antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TIGIT antibody at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:5000 ICC:1:125-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
