WB
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb at 1:5000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.ICC/IF
Immunofluorescence analysis of HeLa treated with TSA(1uM,4hours) and HeLa cells using Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic acetylated peptide around K9 & K14 & K18 & K23 & K27 of human Histone H3 (NP_003520.1). |
| Modification | Acetyl K9/K14/K18/K23/K27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3C1; H3-4 |
|---|---|
| Gene Synonyms | H3/A H3C2 H3C3 H3C4 H3t H3.4 H3/g H3FT H3C16 HIST3H3 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A； |
| Gene Full Name | H3 clustered histone 1;H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A Acetyl-Histone H3-K9/K14/K18/K23/K27 |
| Molecular Weight(MW) | 15kDa( Observed MW 17kDa) |
| Cellular Localization | Chromosome,Nucleus. |
WB
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb at 1:5000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
ICC/IF
Immunofluorescence analysis of HeLa treated with TSA(1uM,4hours) and HeLa cells using Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:1000-1:5000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
