WB
Western blot analysis of Phospho-PKC zeta (T560) on different lysates with Rabbit anti-Phospho-PKC zeta (T560) antibody at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: Mouse eyeball tissue lysate (30 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane) Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-PKC zeta (T560) antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Phospho-PKC zeta (T560) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr560 of Human PKC zeta aa 531-580 / 592. |
| Modification | p-T560 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:2000 IHC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKCZ |
|---|---|
| Gene Synonyms | PKC2 PKC-ZETA |
| Gene Full Name | protein kinase C zeta |
| Gene Summary | Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Alternative Names | 14-3-3-zetaisoform antibody AI098070 antibody aPKCzeta antibody C80388 antibody EC 2.7.11.13 antibody KPCZ_HUMAN antibody nPKC zeta antibody nPKC-zeta antibody OTTHUMP00000001368 antibody OTTHUMP00000044160 antibody 14-3-3-zetaisoform antibody AI098070 antibody aPKCzeta antibody C80388 antibody EC 2.7.11.13 antibody KPCZ_HUMAN antibody nPKC zeta antibody nPKC-zeta antibody OTTHUMP00000001368 antibody OTTHUMP00000044160 antibody PKC 2 antibody PKC ZETA antibody PKC2 antibody Pkcz antibody PKCZETA antibody PKM-zeta included antibody PRKCZ antibody Protein kinase C zeta antibody Protein kinase C zeta form antibody Protein kinase C zeta type antibody r14-3-3 antibody R74924 antibody zetaPKC antibody |
| Molecular Weight(MW) | 68kDa |
| Cellular Localization | Cell junction, Cytoplasm, Endosome, Membrane. |
WB
Western blot analysis of Phospho-PKC zeta (T560) on different lysates with Rabbit anti-Phospho-PKC zeta (T560) antibody at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: Mouse eyeball tissue lysate (30 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane) Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-PKC zeta (T560) antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000-1:2000 IHC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
