WB
Untransfected: Control 293T Lysate (A, D), untreated transfected human PKC Zeta: 293T Lysate (B, E), and transfected human PKC Zeta: 293T Lysate treated with λ protein phosphatase (C, F). Western blot analysis of PKC Zeta phosphorylation in 293T whole cell lysate.IHC
Immunoperoxidase staining was performed on human prostate tissue fixed in formalin and embedded in paraffin, revealing cytoplasmic staining of glandular cells, stromal cells, and smooth muscle cells.IHC
Immunoperoxidase staining of human testicular tissue fixed in formalin and embedded in paraffin reveals cytoplasmic and membrane staining of cells in seminiferous tubules, as well as cytoplasmic staining of Leydig cells.| Product Name | Mouse Monoclonal antibody to p-PKC zeta |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | epitope corresponding to a short amino acid sequence containing Thr 410 phosphorylated PKC z of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCZ |
|---|---|
| Gene Synonyms | PKC2 PKC-ZETA |
| Gene Full Name | protein kinase C zeta |
| Gene Summary | Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 76kDa |
WB
Untransfected: Control 293T Lysate (A, D), untreated transfected human PKC Zeta: 293T Lysate (B, E), and transfected human PKC Zeta: 293T Lysate treated with λ protein phosphatase (C, F). Western blot analysis of PKC Zeta phosphorylation in 293T whole cell lysate.
IHC
Immunoperoxidase staining was performed on human prostate tissue fixed in formalin and embedded in paraffin, revealing cytoplasmic staining of glandular cells, stromal cells, and smooth muscle cells.
IHC
Immunoperoxidase staining of human testicular tissue fixed in formalin and embedded in paraffin reveals cytoplasmic and membrane staining of cells in seminiferous tubules, as well as cytoplasmic staining of Leydig cells.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
