WB
Western blot analysis of FUS/TLS on different lysates with Rabbit anti-FUS/TLS antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: K-562 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FUS/TLS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HepG2 cells labeling FUS/TLS with Rabbit anti-FUS/TLS antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FUS/TLS antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of NIH/3T3 cells labeling FUS/TLS. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | FUS/TLS Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human aa 1-46 / 526. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:50-1:200 ICC:1:100 IF:1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FUS |
|---|---|
| Gene Synonyms | TLS ALS6 ETM4 FUS1 POMP75 altFUS HNRNPP2 |
| Gene Full Name | FUS RNA binding protein |
| Gene Summary | This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009] |
| Alternative Names | 75 kDa DNA pairing protein antibody 75 kDa DNA-pairing protein antibody ALS6 antibody Amyotrophic lateral sclerosis 6 antibody fus antibody FUS CHOP antibody Fus like protein antibody FUS_HUMAN antibody FUS1 antibody Fused in sarcoma antibody 75 kDa DNA pairing protein antibody 75 kDa DNA-pairing protein antibody ALS6 antibody Amyotrophic lateral sclerosis 6 antibody fus antibody FUS CHOP antibody Fus like protein antibody FUS_HUMAN antibody FUS1 antibody Fused in sarcoma antibody Fusion (involved in t(12 16) in malignant liposarcoma) antibody Fusion derived from t(12 16) malignant liposarcoma antibody Fusion gene in myxoid liposarcoma antibody Heterogeneous nuclear ribonucleoprotein P2 antibody hnRNP P2 antibody hnRNPP2 antibody Oncogene FUS antibody Oncogene TLS antibody POMp75 antibody RNA binding protein FUS antibody RNA-binding protein FUS antibody TLS antibody TLS CHOP antibody Translocated in liposarcoma antibody Translocated in liposarcoma protein antibody |
| Molecular Weight(MW) | 53kDa(Observed band size: 70kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of FUS/TLS on different lysates with Rabbit anti-FUS/TLS antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: K-562 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FUS/TLS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HepG2 cells labeling FUS/TLS with Rabbit anti-FUS/TLS antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FUS/TLS antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of NIH/3T3 cells labeling FUS/TLS. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:5000 IHC:1:50-1:200 ICC:1:100 IF:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
