WB
Various lysates were subjected to SDS PAGE followed by western blot with B23/NPM1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody as loading control.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using B23 Antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).| Product Name | B23/NPM1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | B23 Fusion Protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:50000 IHC:1:200-1:1000 ICC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NPM1 |
|---|---|
| Gene Synonyms | B23 NPM |
| Gene Full Name | nucleophosmin 1 |
| Gene Summary | The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017] |
| Alternative Names | B23 NPM1 4F12A3 NPM Nucleophosmin |
| Molecular Weight(MW) | 33kDa |
| Cellular Localization | Cytoplasm,Cytoskeleton,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with B23/NPM1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody as loading control.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using B23 Antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:5000-1:50000 IHC:1:200-1:1000 ICC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
