WB
Western blot analysis of HNF-4-alpha on different lysates with Rabbit anti-HNF-4-alpha antibody at 1/500 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HNF-4-alpha antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Immunofluorescence analysis of paraffin-embedded human colon tissue labeling HNF-4-alpha with Rabbit anti-HNF-4-alpha antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | HNF-4-alpha Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse HNF-4-alpha aa 1-250/474. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCIPWB |
| WB:1:500 IHC:1:400 IF-P:1:200 |
|
| Species Reactivity | HumanMouseRatZebrafish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HNF4A |
|---|---|
| Gene Synonyms | TCF HNF4 MODY FRTS4 MODY1 NR2A1 TCF14 HNF4a7 HNF4a8 HNF4a9 NR2A21 TCF-14 HNF4alpha |
| Gene Full Name | hepatocyte nuclear factor 4 alpha |
| Gene Summary | The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012] |
| Alternative Names | FLJ39654 antibody FRTS4 antibody Hepatic nuclear factor 4 alpha antibody Hepatocyte nuclear factor 4 alpha antibody Hepatocyte nuclear factor 4 antibody Hepatocyte nuclear factor 4-alpha antibody HNF 4 alpha antibody HNF 4 antibody HNF-4-alpha antibody HNF4 antibody FLJ39654 antibody FRTS4 antibody Hepatic nuclear factor 4 alpha antibody Hepatocyte nuclear factor 4 alpha antibody Hepatocyte nuclear factor 4 antibody Hepatocyte nuclear factor 4-alpha antibody HNF 4 alpha antibody HNF 4 antibody HNF-4-alpha antibody HNF4 antibody HNF4A antibody HNF4A_HUMAN antibody HNF4a7 antibody HNF4a8 antibody HNF4a9 antibody Hnf4alpha antibody HNF4alpha10/11/12 antibody MODY 1 antibody MODY antibody MODY1 antibody NR2A1 antibody NR2A21 antibody Nuclear receptor subfamily 2 group A member 1 antibody OTTHUMP00000031060 antibody OTTHUMP00000031062 antibody TCF 14 antibody TCF antibody TCF-14 antibody TCF14 antibody Tcf4 antibody Transcription factor 14 hepatic nuclear factor antibody Transcription factor 14 antibody Transcription factor HNF 4 antibody Transcription factor HNF-4 antibody Transcription factor HNF4 antibody |
| Molecular Weight(MW) | 53kDa(Observed band size: 45~55kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of HNF-4-alpha on different lysates with Rabbit anti-HNF-4-alpha antibody at 1/500 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HNF-4-alpha antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence analysis of paraffin-embedded human colon tissue labeling HNF-4-alpha with Rabbit anti-HNF-4-alpha antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:500 IHC:1:400 IF-P:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
