WB
Western blot analysis of Grp75 on different lysates with Mouse anti-Grp75 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Rat kidney tissue lysate Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Grp75 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of K-562 cells labeling Grp75 with Mouse anti-Grp75 antibody at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Grp75 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.| Product Name | Grp75 Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human Grp75 aa 101-150. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000 IHC:1:500 ICC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSPA9 |
|---|---|
| Gene Synonyms | CSA MOT MOT2 SAAN CRP40 EVPLS GRP75 PBP74 GRP-75 HSPA9B SIDBA4 MTHSP75 HEL-S-124m |
| Gene Full Name | heat shock protein family A (Hsp70) member 9 |
| Gene Summary | This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010] |
| Alternative Names | 75 kDa glucose regulated protein antibody 75 kDa glucose-regulated protein antibody CSA antibody Glucose Regulated Protein antibody Grp 75 antibody GRP-75 antibody GRP75 antibody GRP75_HUMAN antibody Heat shock 70 kDa protein 9 antibody Heat shock 70kD protein 9 antibody 75 kDa glucose regulated protein antibody 75 kDa glucose-regulated protein antibody CSA antibody Glucose Regulated Protein antibody Grp 75 antibody GRP-75 antibody GRP75 antibody GRP75_HUMAN antibody Heat shock 70 kDa protein 9 antibody Heat shock 70kD protein 9 antibody heat shock 70kDa protein 9 antibody Heat shock 70kDa protein 9B antibody Heat shock protein 74 kDa A antibody Heat shock protein A antibody Heat shock protein cognate 74 antibody Hsc74 antibody Hsp74 antibody Hsp74a antibody HSPA9 antibody Hspa9a antibody HSPA9B antibody MGC4500 antibody mitochondrial antibody Mortalin 2 antibody Mortalin antibody Mortalin perinuclear antibody Mortalin2 antibody MOT 2 antibody MOT antibody MOT2 antibody Mthsp70 antibody p66 mortalin antibody P66 MOT antibody PBP74 antibody Peptide binding protein 74 antibody Peptide-binding protein 74 antibody Stress 70 protein mitochondrial antibody Stress 70 protein mitochondrial precursor antibody Stress-70 protein antibody |
| Molecular Weight(MW) | 74kDa |
| Cellular Localization | Mitochondrion. Nucleus. |
WB
Western blot analysis of Grp75 on different lysates with Mouse anti-Grp75 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Rat kidney tissue lysate Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Grp75 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of K-562 cells labeling Grp75 with Mouse anti-Grp75 antibody at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Grp75 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:2000 IHC:1:500 ICC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
