WB
Western blot analysis of lysates from Rat skeletal muscle, using MYH2 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1sWB
Western blot analysis of lysates from U-2 OS cells using MYH2 Rabbit pAb at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60 s.IF-P
Immunofluorescence analysis of paraffin embedded mouse skeletal muscle using MYH2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | MYH2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human MYH2 (NP_001093582.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PWB |
| WB:1:500-1:1000 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYH2 |
|---|---|
| Gene Synonyms | IBM3 CMYO6 CMYP6 MYH2A MYPOP MYHSA2 MYHas8 MyHC-2A MyHC-IIa |
| Gene Full Name | myosin heavy chain 2 |
| Gene Summary | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009] |
| Alternative Names | IBM3 CMYP6 MYH2A MYPOP MYHSA2 MYHas8 MyHC-2A MyHC-IIa MYH2 |
| Molecular Weight(MW) | 223kDa(Observed MW 250kDa) |
| Cellular Localization | Cytoplasm,myofibril. |
WB
Western blot analysis of lysates from Rat skeletal muscle, using MYH2 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s
WB
Western blot analysis of lysates from U-2 OS cells using MYH2 Rabbit pAb at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60 s.
IF-P
Immunofluorescence analysis of paraffin embedded mouse skeletal muscle using MYH2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
