WB
Immunoblotting of U251-cell lysate showing reactivity with Nestin Antibody.ICC/IF
Immunostaining of intracellular Nestin filaments in U251 cells.| Product Name | Nestin (NES) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A 150-amino-acid fragment from the cloned Human Nestin, conjugated to Glutathione S-Transferase. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NES |
|---|---|
| Gene Synonyms | Nbla00170 |
| Gene Full Name | nestin |
| Gene Summary | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011] |
| Molecular Weight(MW) | 150-300kDa |
| Cellular Localization | Cytoplasm, Intermediate filament. |
WB
Immunoblotting of U251-cell lysate showing reactivity with Nestin Antibody.
ICC/IF
Immunostaining of intracellular Nestin filaments in U251 cells.| Application Notes | WB:1:1000-1:5000 ICC:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Storage Buffer | PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide |
Data sheet for OM643249