WB
Western blot analysis of NeuroD1 on different lysates with Rabbit anti-NeuroD1 antibody at 1/1,000 dilution. Lane 1: Mouse cerebellum (P7) tissue lysate (40 µg/Lane), Lane 2: Mouse cerebellum tissue lysate (no heat) (40 µg/Lane), Lane 3: Mouse liver tissue lysate (negative) (40 µg/Lane), Lane 4: Rat cerebellum tissue lysate (40 µg/Lane), Lane 5: Rat liver tissue lysate (negative) (40 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 51 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NeuroD1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Immunofluorescence analysis of frozen rat cerebellum tissue with Rabbit anti-NeuroD1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | NeuroD1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human NeuroD1 aa 1-44 / 356. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:500-1:1000 IHC:1:200-1:500 IF-F:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NEUROD1 |
|---|---|
| Gene Synonyms | T2D BETA2 BHF-1 MODY6 NEUROD bHLHa3 |
| Gene Full Name | neuronal differentiation 1 |
| Gene Summary | This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008] |
| Alternative Names | atonal antibody basic helix loop helix transcription factor antibody BETA 2 antibody Beta cell E box transactivator 2 antibody BETA2 antibody BHF 1 antibody BHF1 antibody bHLHa3 antibody class A basic helix loop helix protein 3 antibody Class A basic helix-loop-helix protein 3 antibody atonal antibody basic helix loop helix transcription factor antibody BETA 2 antibody Beta cell E box transactivator 2 antibody BETA2 antibody BHF 1 antibody BHF1 antibody bHLHa3 antibody class A basic helix loop helix protein 3 antibody Class A basic helix-loop-helix protein 3 antibody MODY 6 antibody MODY6 antibody NDF1_HUMAN antibody NeuroD antibody NeuroD1 antibody Neurogenic differentiation 1 antibody Neurogenic differentiation factor 1 antibody neurogenic helix loop helix protein NEUROD antibody Neuronal differentiation 1 antibody |
| Molecular Weight(MW) | 40kDa(Observed band size: 50kDa) |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of NeuroD1 on different lysates with Rabbit anti-NeuroD1 antibody at 1/1,000 dilution. Lane 1: Mouse cerebellum (P7) tissue lysate (40 µg/Lane), Lane 2: Mouse cerebellum tissue lysate (no heat) (40 µg/Lane), Lane 3: Mouse liver tissue lysate (negative) (40 µg/Lane), Lane 4: Rat cerebellum tissue lysate (40 µg/Lane), Lane 5: Rat liver tissue lysate (negative) (40 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 51 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NeuroD1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Immunofluorescence analysis of frozen rat cerebellum tissue with Rabbit anti-NeuroD1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:500-1:1000 IHC:1:200-1:500 IF-F:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
