WB
Western blot analysis of YTHDF1 on different lysates with Rabbit anti-YTHDF1 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: MCF-7 cell lysate (10 µg/Lane) Lane 5: NIH/3T3 cell lysate (10 µg/Lane) Lane 6: PC-12 cell lysate (10 µg/Lane) Lane 7: HCT116 cell lysate (10 µg/Lane) Lane 8: Mouse testis liver tissue lysate (20 µg/Lane) Lane 9: Rat testis tissue lysate (20 µg/Lane) Lane 10: Mouse brain tissue lysate (20 µg/Lane) Lane 11: Rat brain tissue lysate (20 µg/Lane) Exposure time: 5 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-YTHDF1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of 293 cells labeling YTHDF1. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | YTHDF1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human YTHDF1 aa 300-559. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:1000 IHC:1:1000 FC:1ug/ml |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | YTHDF1 |
|---|---|
| Gene Synonyms | DF1 C20orf21 |
| Gene Full Name | YTH N6-methyladenosine RNA binding protein F1 |
| Gene Summary | Enables N6-methyladenosine-containing RNA reader activity and ribosome binding activity. Involved in mRNA destabilization; positive regulation of translational initiation; and stress granule assembly. Located in P-body and cytoplasmic stress granule. Implicated in colorectal cancer. [provided by Alliance of Genome Resources, Nov 2024] |
| Alternative Names | C20orf21 antibody DACA 1 antibody DACA-1 antibody Dermatomyositis associated with cancer putative autoantigen 1 antibody YTH domain family 1 antibody YTH domain family member 1 antibody YTH domain family protein 1 antibody YTHD1 antibody YTHD1_HUMAN antibody Ythdf1 antibody |
| Molecular Weight(MW) | 61kDa(Observed band size: 69kDa) |
| Cellular Localization | Cytoplasm, P-body. |
WB
Western blot analysis of YTHDF1 on different lysates with Rabbit anti-YTHDF1 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: MCF-7 cell lysate (10 µg/Lane) Lane 5: NIH/3T3 cell lysate (10 µg/Lane) Lane 6: PC-12 cell lysate (10 µg/Lane) Lane 7: HCT116 cell lysate (10 µg/Lane) Lane 8: Mouse testis liver tissue lysate (20 µg/Lane) Lane 9: Rat testis tissue lysate (20 µg/Lane) Lane 10: Mouse brain tissue lysate (20 µg/Lane) Lane 11: Rat brain tissue lysate (20 µg/Lane) Exposure time: 5 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-YTHDF1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of 293 cells labeling YTHDF1. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:1000 FC:1ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
