WB
Western blot analysis of Rat brain, using C10orf32 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.ICC/IF
Immunofluorescence analysis of MCF-7 cells using C10orf32 antibody. Blue: DAPI for nuclear staining.| Product Name | C10orf32 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human C10orf32 (NP_653192.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BORCS7 |
|---|---|
| Gene Synonyms | C10orf32 |
| Gene Full Name | BLOC-1 related complex subunit 7 |
| Gene Summary | Predicted to act upstream of or within several processes, including lysosome localization; motor behavior; and response to cycloheximide. Part of BORC complex. [provided by Alliance of Genome Resources, Nov 2024] |
| Alternative Names | C10orf32 |
| Molecular Weight(MW) | 12kDa( Observed MW 18kDa) |
| Cellular Localization | Lysosome membrane. |
WB
Western blot analysis of Rat brain, using C10orf32 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of MCF-7 cells using C10orf32 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB:1:1000-1:5000 ICC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
