WB
Western blot analysis of Integrin alpha 2 on different lysates with Rabbit anti-Integrin alpha 2 antibody at 1/5,000 dilution. Lane 1: MCF7 cell lysate, Lane 2: Mouse spleen tissue lysate, Lane 3: Rat spleen tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of A431 cells labeling Integrin alpha 2 with Rabbit anti-Integrin alpha 2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Integrin alpha 2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of A431 cells labeling Integrin alpha 2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
Integrin alpha 2 was immunoprecipitated in 0.2mg MCF7 cell lysate with Rabbit anti-Integrin alpha 2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Integrin alpha 2 antibody at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input), Lane 2: Rabbit IgG instead of Rabbit anti-Integrin alpha 2 antibody in MCF7 cell lysate, Lane 3: Rabbit anti-Integrin alpha 2 antibody IP in MCF7 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 43 seconds.| Product Name | Integrin alpha 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Integrin alpha 2 aa 1,132-1,181 / 1,181. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000 IHC:1:50-1:200 ICC:1:100-1:500 FC:1:1000 IP:Use at an assay dependent concentration. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ITGA2 |
|---|---|
| Gene Synonyms | BR GPIa CD49B HPA-5 VLA-2 VLAA2 |
| Gene Full Name | integrin subunit alpha 2 |
| Gene Summary | This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Alternative Names | BR antibody CD 49b antibody CD49 antigen like family member B antibody CD49 antigen-like family member B antibody CD49b antibody CD49b antigen antibody Collagen receptor antibody DX5 antibody Glycoprotein Ia deficiency included antibody GP Ia antibody BR antibody CD 49b antibody CD49 antigen like family member B antibody CD49 antigen-like family member B antibody CD49b antibody CD49b antigen antibody Collagen receptor antibody DX5 antibody Glycoprotein Ia deficiency included antibody GP Ia antibody GP Ia deficiency included antibody GPIa antibody HPA 5 included antibody HPA5 included antibody Human platelet alloantigen system 5 antibody Integrin alpha 2 antibody Integrin alpha-2 antibody Integrin alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody ITA2_HUMAN antibody ITGA2 antibody Platelet alloantigen Br(a) included antibody Platelet antigen Br antibody Platelet glycoprotein GPIa antibody Platelet glycoprotein Ia antibody Platelet glycoprotein Ia/IIa antibody Platelet membrane glycoprotein Ia antibody Platelet receptor for collagen deficiency of included antibody Very late activation protein 2 receptor alpha 2 subunit antibody VLA 2 alpha chain antibody VLA 2 antibody VLA 2 subunit alpha antibody VLA-2 subunit alpha antibody VLA2 antibody VLA2 receptor alpha 2 subunit antibody VLAA2 antibody |
| Molecular Weight(MW) | 129kDa(Observed band size:150kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of Integrin alpha 2 on different lysates with Rabbit anti-Integrin alpha 2 antibody at 1/5,000 dilution. Lane 1: MCF7 cell lysate, Lane 2: Mouse spleen tissue lysate, Lane 3: Rat spleen tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of A431 cells labeling Integrin alpha 2 with Rabbit anti-Integrin alpha 2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Integrin alpha 2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of A431 cells labeling Integrin alpha 2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
Integrin alpha 2 was immunoprecipitated in 0.2mg MCF7 cell lysate with Rabbit anti-Integrin alpha 2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Integrin alpha 2 antibody at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input), Lane 2: Rabbit IgG instead of Rabbit anti-Integrin alpha 2 antibody in MCF7 cell lysate, Lane 3: Rabbit anti-Integrin alpha 2 antibody IP in MCF7 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 43 seconds.| Application Notes | WB:1:5000 IHC:1:50-1:200 ICC:1:100-1:500 FC:1:1000 IP:Use at an assay dependent concentration. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
