WB
Western blot analysis of various lysates using Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffin embedded Human lung cancer using Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.IHC
Immunohistochemistry analysis of paraffin embedded Human tonsil using Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.ICC/IF
Immunofluorescence analysis of U2OS cells using Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb at dilution of 1:100. Secondary antibody: Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 30-162 of human Integrin alpha 2 (ITGA2/CD49b) (NP_002194.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITGA2 |
|---|---|
| Gene Synonyms | BR GPIa CD49B HPA-5 VLA-2 VLAA2 |
| Gene Full Name | integrin subunit alpha 2 |
| Gene Summary | This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Alternative Names | BR GPIa CD49B HPA-5 VLA-2 VLAA2 b) |
| Molecular Weight(MW) | 129kDa(Observed MW 150kDa) |
| Cellular Localization | Membrane,Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffin embedded Human lung cancer using Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
IHC
Immunohistochemistry analysis of paraffin embedded Human tonsil using Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ICC/IF
Immunofluorescence analysis of U2OS cells using Integrin alpha 2 (ITGA2/CD49b) Rabbit pAb at dilution of 1:100. Secondary antibody: Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
