WB
Panc-1(Human) Cell Lysate at 30 ug Lovo(Human) Cell Lysate at 30 ug Thymus (Mouse) Lysate at 40 ug MKN45(Human) Cell Lysate at 30 ug Primary: Anti-MUC4 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 120 kD.IHC
Paraformaldehyde-fixed, paraffin embedded (Human oral squamous cell carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Muc4) Polyclonal Antibody at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.| Product Name | MUC4 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human Mucin-4 beta chain: 1501-1613/2169 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MUC4 |
|---|---|
| Gene Synonyms | ASGP MUC-4 HSA276359 |
| Gene Full Name | mucin 4, cell surface associated |
| Gene Summary | The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008] |
| Alternative Names | ASGP HSA276359 MUC-4 4933405I11Rik MUC4_HUMAN MUC4 Ascites sialoglycoprotein (ASGP) Pancreatic adenocarcinoma mucin Testis mucin Tracheobronchial mucin MUC4_MOUSE mucin 4 cell surface associated mucin 4 tracheobronchial ascites sialoglycoprotein |
| Molecular Weight(MW) | 80kDa(Observed band size: 120kD) |
| Cellular Localization | Cell membrane,Secreted. |
WB
Panc-1(Human) Cell Lysate at 30 ug Lovo(Human) Cell Lysate at 30 ug Thymus (Mouse) Lysate at 40 ug MKN45(Human) Cell Lysate at 30 ug Primary: Anti-MUC4 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 120 kD.
IHC
Paraformaldehyde-fixed, paraffin embedded (Human oral squamous cell carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Muc4) Polyclonal Antibody at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
