DB
Dot blot analysis of anti-RYR2 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR2 antibody was diluted with 1/500.IHC
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin.ICC/IF
ICC staining RYR2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.FC
Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | RYR2 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human RYR2 aa 1,471-1,520 / 4,967. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBFCICC/IFIHC |
| DB:1:500 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RYR2 |
|---|---|
| Gene Synonyms | RyR ARVC2 ARVD2 RYR-2 VTSIP VACRDS |
| Gene Full Name | ryanodine receptor 2 |
| Gene Summary | This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008] |
| Alternative Names | Arrhythmogenic right ventricular dysplasia 2 antibody ARVC2 antibody ARVD2 antibody Brain ryanodine receptor calcium release channel antibody Brain type ryanodine receptor antibody Cardiac muscle ryanodine receptor calcium release channel antibody Cardiac muscle type ryanodine receptor antibody CCO antibody Central core disease of muscle antibody HBRR antibody Arrhythmogenic right ventricular dysplasia 2 antibody ARVC2 antibody ARVD2 antibody Brain ryanodine receptor calcium release channel antibody Brain type ryanodine receptor antibody Cardiac muscle ryanodine receptor calcium release channel antibody Cardiac muscle type ryanodine receptor antibody CCO antibody Central core disease of muscle antibody HBRR antibody hRYR 2 antibody hRYR2 antibody MHS antibody MHS1 antibody Ryanodine receptor 1 (skeletal) antibody Ryanodine receptor 1 antibody Ryanodine receptor 2 (cardiac) antibody Ryanodine receptor 2 antibody Ryanodine receptor 3 antibody Ryanodine receptor type1 antibody RYDR antibody RYR 1 antibody RYR 2 antibody RYR 3 antibody RYR antibody RYR-1 antibody RyR1 antibody RYR1_HUMAN antibody RYR2 antibody RYR3 antibody Sarcoplasmic reticulum calcium release channel antibody Skeletal muscle calcium release channel antibody Skeletal muscle ryanodine receptor antibody Skeletal muscle type ryanodine receptor antibody Skeletal muscle-type ryanodine receptor antibody SKRR antibody Type 1 like ryanodine receptor antibody Type 1 ryanodine receptor antibody VTSIP antibody |
| Molecular Weight(MW) | 565kDa |
| Cellular Localization | Membrane. Sarcoplasmic reticulum. |
DB
Dot blot analysis of anti-RYR2 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR2 antibody was diluted with 1/500.
IHC
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin.
ICC/IF
ICC staining RYR2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
FC
Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Application Notes | DB:1:500 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
