WB
Western blot analysis of Ryanodine Receptor 2 (Phospho-S2808) expression in U251 (A) whole cell lysates.IHC
Immunohistochemical analysis of Ryanodine Receptor 2 (Phospho-S2808) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.ICC/IF
Immunofluorescent analysis of Ryanodine Receptor 2 (Phospho-S2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Product Name | Anti-Ryanodine Receptor 2 (Phospho-S2808) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S2808 of human Ryanodine Receptor 2 protein. The exact sequence is proprietary. |
| Modification | p-S2808 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RYR2 |
|---|---|
| Gene Synonyms | RyR ARVC2 ARVD2 RYR-2 VTSIP VACRDS |
| Gene Full Name | ryanodine receptor 2 |
| Gene Summary | This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008] |
| Alternative Names | Ryanodine receptor 2 RYR-2 RyR2 hRYR-2 Cardiac muscle ryanodine receptor Cardiac muscle ryanodine receptor-calcium release channel Type 2 ryanodine receptor |
| Molecular Weight(MW) | 564 kDa(Observed band size: 565 kDa) |
| Cellular Localization | Membrane,Sarcoplasmic reticulum |
WB
Western blot analysis of Ryanodine Receptor 2 (Phospho-S2808) expression in U251 (A) whole cell lysates.
IHC
Immunohistochemical analysis of Ryanodine Receptor 2 (Phospho-S2808) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ICC/IF
Immunofluorescent analysis of Ryanodine Receptor 2 (Phospho-S2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
