WB
Western blot analysis of LSM8 using anti-LSM8 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: Rat testis tissue lysates, Lane 6: Rat pancrease tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-LSM8 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for LSM8 at approximately 16 kDa. The expected band size for LSM8 is at 10 kDa.IHC
IHC analysis of LSM8 using anti-LSM8 antibody. LSM8 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-LSM8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
IF analysis of LSM8 using anti-LSM8 antibody. LSM8 was detected in an immunocytochemical section of Hela cells. The section was incubated with mouse anti-LSM8 Antibody at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Jurkat cells using anti-LSM8 antibody. Overlay histogram showing Jurkat cells stained withanti-LSM8 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-LSM8 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Mouse monoclonal antibody to LSM8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human LSM8 recombinant protein (Position: M1-H96). Human LSM8 shares 100% amino acid (aa) sequence identity with both mouse and rat LSM8. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Symbol | LSM8 |
|---|---|
| Gene Synonyms | NAA38 |
| Gene Full Name | LSM8 homolog, U6 small nuclear RNA associated |
| Gene Summary | This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus. [provided by RefSeq, Jan 2010] |
| Alternative Names | LSM8 NAA38 YJR022W |
| Molecular Weight(MW) | 10kDa( Observed MW 16kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of LSM8 using anti-LSM8 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: Rat testis tissue lysates, Lane 6: Rat pancrease tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-LSM8 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for LSM8 at approximately 16 kDa. The expected band size for LSM8 is at 10 kDa.
IHC
IHC analysis of LSM8 using anti-LSM8 antibody. LSM8 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-LSM8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
IF analysis of LSM8 using anti-LSM8 antibody. LSM8 was detected in an immunocytochemical section of Hela cells. The section was incubated with mouse anti-LSM8 Antibody at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Jurkat cells using anti-LSM8 antibody. Overlay histogram showing Jurkat cells stained withanti-LSM8 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-LSM8 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
