WB
Various lysates were subjected to SDS PAGE followed by western blot with GPX4 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.IHC
Immunohistochemical analysis of paraffin embedded human liver cancer tissue slide using GPX4 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody at dilution of 1:800 and CoraLite®488 Conjugated Goat Anti-Mouse IgG(H+L).| Product Name | GPX4 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | GPX4 Fusion Protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:4000 IHC:1:1000-1:4000 ICC:1:400-1:1600 |
|
| Species Reactivity | ChickenDogHumanMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GPX4 |
|---|---|
| Gene Synonyms | MCSP SMDS GPx-4 PHGPx snGPx GSHPx-4 snPHGPx |
| Gene Full Name | glutathione peroxidase 4 |
| Gene Summary | The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. [provided by RefSeq, Dec 2018] |
| Alternative Names | 3F5G5 EC:1.11.1.12 EC:1.11.1.9 GPx 4 GPx-4 |
| Molecular Weight(MW) | 20-23kDa |
| Cellular Localization | Cytoplasm,Mitochondrion. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with GPX4 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.
IHC
Immunohistochemical analysis of paraffin embedded human liver cancer tissue slide using GPX4 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody at dilution of 1:800 and CoraLite®488 Conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:1000-1:4000 IHC:1:1000-1:4000 ICC:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
