ICC/IF
Confocal imaging of paraffin-embedded Mouse small intestine tissue using Alexa Fluor® 647 Rabbit anti-Mouse CD326/EpCAM mAb (dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. objective: 40x.FC
Flow cytometry: 1X10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface stained with Alexa Fluor® 647 Rabbit anti-Mouse CD326/EpCAM mAb (5ul/Test,orange line) or Alexa Fluor® 647 Rabbit IgG isotype control (5ul/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).| Product Name | Alexa Fluor® 647 Rabbit anti-Mouse CD326/EpCAM mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 24-266 of mouse CD326/EpCAM (NP_032558.2). |
| Conjugation | Alexa Fluor? 647 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IF |
| ICC:1:50-1:200 FC:5ul per 10^6 cells in 100 μl volume |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Epcam |
|---|---|
| Gene Synonyms | EGP Ly74 gp40 CD326 EGP-2 TROP1 Egp314 Ep-CAM EpCAM1 Tacsd1 GA733-2 Tacstd1 |
| Gene Full Name | epithelial cell adhesion molecule |
| Gene Summary | Predicted to enable cadherin binding activity involved in cell-cell adhesion. Involved in ureteric bud development. Located in cell surface. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Used to study congenital diarrhea 5 with tufting enteropathy. Human ortholog(s) of this gene implicated in congenital diarrhea 5 with tufting enteropathy and hereditary nonpolyposis colorectal cancer type 8. Orthologous to human EPCAM (epithelial cell adhesion molecule). [provided by Alliance of Genome Resources, Nov 2024] |
| Cellular Localization | Lateral cell membrane. |
ICC/IF
Confocal imaging of paraffin-embedded Mouse small intestine tissue using Alexa Fluor® 647 Rabbit anti-Mouse CD326/EpCAM mAb (dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. objective: 40x.
FC
Flow cytometry: 1X10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface stained with Alexa Fluor® 647 Rabbit anti-Mouse CD326/EpCAM mAb (5ul/Test,orange line) or Alexa Fluor® 647 Rabbit IgG isotype control (5ul/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).| Application Notes | ICC:1:50-1:200 FC:5ul per 10^6 cells in 100 μl volume |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8℃. Avoid freeze. |
| Storage Buffer | Buffer: PBS with 0.03% proclin300,0.2% BSA,pH7.3. |
Data sheet for OM643339