WB
Western blot analysis of extracts from various samples, using ORMDL3 Antibody. Lane 1: Mcf7 cells(serum starvation treatment), blocked with antigen-specific peptides. Lane 2: Mcf7 cells(serum starvation treatment). Lane 3: K562 cells(uv treatment).ICC/IF
ORMDL3 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to ORMDL3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from Human ORMDL3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ORMDL3 |
|---|---|
| Gene Full Name | ORMDL sphingolipid biosynthesis regulator 3 |
| Gene Summary | Involved in ceramide metabolic process. Acts upstream of or within several processes, including negative regulation of B cell apoptotic process; negative regulation of ceramide biosynthetic process; and positive regulation of protein localization to nucleus. Located in endoplasmic reticulum. Part of serine palmitoyltransferase complex. [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | ORM1 (S. cerevisiae)-like 3 ORM1-like 3 (S. cerevisiae) ORM1-like 3 ORM1-like protein 3 ORMDL sphingolipid biosynthesis regulator 3 Ormdl3 ORML3_HUMAN OTTHUMP00000164334 |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Endoplasmic reticulum membrane>Multi-pass membrane protein. |
WB
Western blot analysis of extracts from various samples, using ORMDL3 Antibody. Lane 1: Mcf7 cells(serum starvation treatment), blocked with antigen-specific peptides. Lane 2: Mcf7 cells(serum starvation treatment). Lane 3: K562 cells(uv treatment).
ICC/IF
ORMDL3 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
