WB
Western blot analysis of Phospho-STING (S366) on different lysates with Rabbit anti-Phospho-STING (S366) antibody at 1/2,000 dilution. Lane 1: THP-1 treated with 80nM TPA for 16 hours cell lysate, Lane 2: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, Lane 3: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, then the membrane treated with λpp for 1 hour. Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.DB
Dot blot analysis of Phospho-STING (S366) on different peptides with Rabbit anti-Phospho-STING (S366) antibody at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified STING peptide (negative) Lane 2: Phospho-STING (S366) peptide (positive) Proteins loading: 100ng, 50ng, 10ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute.IP
Phospho-STING (S366) was immunoprecipitated from 0.2 mg THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate with Rabbit anti-Phospho-STING (S366) antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Phospho-STING (S366) antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate (input), Lane 2:Rabbit anti-Phospho-STING (S366) antibody IP in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Phospho-STING (S366) antibody in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 10 seconds.| Product Name | Phospho-STING (S366) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser366 of Human STING. |
| Modification | p-S366 |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Alligator |
| Tested Applications | DBIPWB |
| WB:1:2000 DB:1:2000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | STING1 |
|---|---|
| Gene Synonyms | ERIS MITA MPYS SAVI NET23 STING hMITA hSTING TMEM173 STING-beta |
| Gene Full Name | stimulator of interferon response cGAMP interactor 1 |
| Gene Summary | This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
| Alternative Names | endoplasmic reticulum IFN stimulator antibody Endoplasmic reticulum interferon stimulator antibody ERIS antibody FLJ38577 antibody hMITA antibody hSTING antibody Mediator of IRF3 activation antibody MITA antibody Mitochondrial mediator of IRF3 activation antibody MPYS antibody endoplasmic reticulum IFN stimulator antibody Endoplasmic reticulum interferon stimulator antibody ERIS antibody FLJ38577 antibody hMITA antibody hSTING antibody Mediator of IRF3 activation antibody MITA antibody Mitochondrial mediator of IRF3 activation antibody MPYS antibody N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody NET23 antibody Stimulator of interferon genes antibody Stimulator of interferon genes protein antibody STING antibody TM173_HUMAN antibody Tmem173 antibody Transmembrane protein 173 antibody |
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Endoplasmic reticulum membrane, Cytoplasm, perinuclear region, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Mitochondrion outer membrane, Cell membrane. |
WB
Western blot analysis of Phospho-STING (S366) on different lysates with Rabbit anti-Phospho-STING (S366) antibody at 1/2,000 dilution. Lane 1: THP-1 treated with 80nM TPA for 16 hours cell lysate, Lane 2: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, Lane 3: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, then the membrane treated with λpp for 1 hour. Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
DB
Dot blot analysis of Phospho-STING (S366) on different peptides with Rabbit anti-Phospho-STING (S366) antibody at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified STING peptide (negative) Lane 2: Phospho-STING (S366) peptide (positive) Proteins loading: 100ng, 50ng, 10ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute.
IP
Phospho-STING (S366) was immunoprecipitated from 0.2 mg THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate with Rabbit anti-Phospho-STING (S366) antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Phospho-STING (S366) antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate (input), Lane 2:Rabbit anti-Phospho-STING (S366) antibody IP in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Phospho-STING (S366) antibody in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 10 seconds.| Application Notes | WB:1:2000 DB:1:2000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
