WB
Western blot analysis of ASH2L on different lysates with Rabbit anti-ASH2L antibody at 1/500 dilution. Lane 1: PC-12 cell lysate, Lane 2: Hela cell lysate, Lane 3: 293T cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ASH2L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining ASH2L (1/500) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | ASH2L Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human ASH2L aa 533-628 / 628. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:500 ICC:1:200-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ASH2L |
|---|---|
| Gene Synonyms | ASH2 Bre2 ASH2L1 ASH2L2 |
| Gene Full Name | ASH2 like, histone lysine methyltransferase complex subunit |
| Gene Summary | Enables beta-catenin binding activity and transcription cis-regulatory region binding activity. Involved in positive regulation of cell population proliferation; response to estrogen; and transcription initiation-coupled chromatin remodeling. Acts upstream of or within DNA damage response. Located in nucleus. Part of MLL1 complex; MLL3/4 complex; and Set1C/COMPASS complex. [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | ASH 2 antibody Ash2 (absent small or homeotic) like antibody Ash2 (absent small or homeotic) like (Drosophila) antibody ASH2 antibody ASH2 LIKE antibody ASH2 like protein antibody ASH2-like protein antibody Ash2l antibody ASH2L_HUMAN antibody ASH2L1 antibody ASH 2 antibody Ash2 (absent small or homeotic) like antibody Ash2 (absent small or homeotic) like (Drosophila) antibody ASH2 antibody ASH2 LIKE antibody ASH2 like protein antibody ASH2-like protein antibody Ash2l antibody ASH2L_HUMAN antibody ASH2L1 antibody ASH2L2 antibody Bre 2 antibody Bre2 antibody Discs 2-like antibody Drosophila absent small or homeotic antibody Drosophila ASH2-like antibody Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody |
| Molecular Weight(MW) | 69kDa(Observed band size: 75 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of ASH2L on different lysates with Rabbit anti-ASH2L antibody at 1/500 dilution. Lane 1: PC-12 cell lysate, Lane 2: Hela cell lysate, Lane 3: 293T cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ASH2L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining ASH2L (1/500) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:500 ICC:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
