WB
Western blot analysis of various lysates using ASH2L Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.IHC
Immunohistochemistry analysis of ASH2L in paraffin-embedded human colon carcinoma tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of C2C12 cells using ASH2L Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488 conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). objective: 100x.IF-P
Confocal imaging of paraffin-embedded Mouse colon tissue using ASH2L Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Product Name | ASH2L Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 529-628 of human ASH2L (Q9UBL3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:50-1:200 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASH2L |
|---|---|
| Gene Synonyms | ASH2 Bre2 ASH2L1 ASH2L2 |
| Gene Full Name | ASH2 like, histone lysine methyltransferase complex subunit |
| Gene Summary | Enables beta-catenin binding activity and transcription cis-regulatory region binding activity. Involved in positive regulation of cell population proliferation; response to estrogen; and transcription initiation-coupled chromatin remodeling. Acts upstream of or within DNA damage response. Located in nucleus. Part of MLL1 complex; MLL3/4 complex; and Set1C/COMPASS complex. [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | ASH2 Bre2 ASH2L1 ASH2L2 ASH2L |
| Molecular Weight(MW) | 69kDa(Observed MW 69kDa/85kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of various lysates using ASH2L Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
IHC
Immunohistochemistry analysis of ASH2L in paraffin-embedded human colon carcinoma tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of C2C12 cells using ASH2L Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488 conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). objective: 100x.
IF-P
Confocal imaging of paraffin-embedded Mouse colon tissue using ASH2L Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:50-1:200 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
