WB
Western blot analysis of xCT/SLC7A11 on different lysates with Mouse anti-xCT/SLC7A11 antibody at 1/500 dilution. Lane 1: HT-29 cell lysate, Lane 2: A549 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT/SLC7A11 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HT-29 cells labeling xCT/SLC7A11 with Mouse anti-xCT/SLC7A11 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-xCT/SLC7A11 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HT-29 cells labeling xCT/SLC7A11. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | xCT/SLC7A11 Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | 293 cell line overexpressing SLC7A11. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:200 ICC:1:100-1:200 FC:1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC7A11 |
|---|---|
| Gene Synonyms | xCT CCBR1 |
| Gene Full Name | solute carrier family 7 member 11 |
| Gene Summary | This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death. [provided by RefSeq, Sep 2011] |
| Alternative Names | Amino acid transport system xc xCT antibody antibody Amino acid transport system xc- antibody Calcium channel blocker resistance protein CCBR1 antibody Calcium channel blocker resistance protein CCBR1 antibody antibody CCBR1 antibody Cysteine/glutamate transporter antibody antibody Cystine/glutamate transporter antibody OTTHUMP00000164578 antibody SLC7A11 antibody Solute carrier family 7 (anionic amino acid transporter light chain xc- system) member 11 antibody Amino acid transport system xc xCT antibody antibody Amino acid transport system xc- antibody Calcium channel blocker resistance protein CCBR1 antibody Calcium channel blocker resistance protein CCBR1 antibody antibody CCBR1 antibody Cysteine/glutamate transporter antibody antibody Cystine/glutamate transporter antibody OTTHUMP00000164578 antibody SLC7A11 antibody Solute carrier family 7 (anionic amino acid transporter light chain xc- system) member 11 antibody solute carrier family 7 antibody Solute carrier family 7 member 11 antibody Solute carrier family 7 (cationic amino acid transporter y+ system) member 11 antibody SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody xCT antibody XCT_HUMAN antibody |
| Molecular Weight(MW) | 55kDa |
| Cellular Localization | Cell membrane, Cell projection, microvillus membrane. |
WB
Western blot analysis of xCT/SLC7A11 on different lysates with Mouse anti-xCT/SLC7A11 antibody at 1/500 dilution. Lane 1: HT-29 cell lysate, Lane 2: A549 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT/SLC7A11 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HT-29 cells labeling xCT/SLC7A11 with Mouse anti-xCT/SLC7A11 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-xCT/SLC7A11 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HT-29 cells labeling xCT/SLC7A11. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:5000 IHC:1:200 ICC:1:100-1:200 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
