WB
HepG2 cells were subjected to SDS PAGE followed by western blot with RBPJ antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using RBPJ antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-RBPJ (IP:4ug; Detection: 1:1000) with HepG2 cells lysate 1000 ug.| Product Name | RBPJ Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RBPJ protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:500 IP:0.5-4.0ug for 1.0-3.0mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.45mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBPJ |
|---|---|
| Gene Synonyms | SUH csl AOS3 CBF1 KBF2 CBF-1 RBP-J RBPJK IGKJRB RBP-JK RBPSUH IGKJRB1 RBP-J kappa |
| Gene Full Name | recombination signal binding protein for immunoglobulin kappa J region |
| Gene Summary | The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013] |
| Alternative Names | CBF 1 CBF1 csl IGKJRB IGKJRB1 KBF2 RBP J RBP J kappa RBP JK RBPJ RBPJK RBPSUH SUH |
| Molecular Weight(MW) | 56kDa(Observed Molecular Weight 60kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
HepG2 cells were subjected to SDS PAGE followed by western blot with RBPJ antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using RBPJ antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-RBPJ (IP:4ug; Detection: 1:1000) with HepG2 cells lysate 1000 ug.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 IP:0.5-4.0ug for 1.0-3.0mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
