RBPSUH is a DNA binding protein that activates a full transcriptional response but only demonstrates partial anti-apoptotic activity. RTA-mediated redirection of RBPSUH activity from repression to activation is critical for lytic viral replication.1) Subramanyam, D. (2006) Virology 347 (1), 191-198.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RBPJ.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
RBPJ antibody can be used for detection of RBPJ by ELISA at 1:312500. RBPJ antibody can be used for detection of RBPJ by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Liver cell lysates.
Application
Antibody used in IHC on Human Stomach.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
RBPJ antibody can be used for detection of RBPJ by ELISA at 1:312500. RBPJ antibody can be used for detection of RBPJ by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RBPJ antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.