WB
Various lysates were subjected to SDS PAGE followed by western blot with ABL1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ABL1 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).FC
1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ABL and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | ABL1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ABL1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFWB |
| WB:1:2000-1:10000 ICC:1:400-1:1600 FC: FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ABL1 |
|---|---|
| Gene Synonyms | ABL JTK7 p150 c-ABL v-abl CHDSKM c-ABL1 BCR-ABL bcr/abl |
| Gene Full Name | ABL proto-oncogene 1, non-receptor tyrosine kinase |
| Gene Summary | This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014] |
| Alternative Names | c ABL 1E7A5 Abelson tyrosine-protein kinase 1 ABL EC:2.7.10.2 |
| Molecular Weight(MW) | 123kDa(Observed Molecular Weight 140kDa) |
| Cellular Localization | Cytoplasm, cytoskeleton, Nucleus, Mitochondrion; Nucleus membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ABL1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ABL1 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC
1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ABL and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:2000-1:10000 ICC:1:400-1:1600 FC: FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
