WB
Western blot analysis of various lysates using RBM3 Rabbit mAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse colon tissue using RBM3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of HeLa cells using RBM3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | RBM3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 58-157 of human RBM3 (P98179). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBM3 |
|---|---|
| Gene Synonyms | RNPL IS1-RNPL |
| Gene Full Name | RNA binding motif protein 3 |
| Gene Summary | This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008] |
| Alternative Names | RNPL IS1-RNPL RBM3 |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Cell projection,Cytoplasm,Nucleus,dendrite. |
WB
Western blot analysis of various lysates using RBM3 Rabbit mAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse colon tissue using RBM3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of HeLa cells using RBM3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
