Application
Western blot analysis of RBM3 antibody (Center) (Cat.# AP7397c) in NIH-3T3 cell line lysates (35ug/lane). RBM3 (arrow) was detected using the purified Pab.Application
RBM3 Antibody (Center) (RB18776) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RBM3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RBM3 Antibody (Center) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of RBM3 (arrow) using rabbit polyclonal RBM3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM3 gene.| Product Name | RBM3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBM3; RNPL; Putative RNA-binding protein 3; RNA-binding motif protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNPL |
|---|---|
| Alternative Names | RBM3 RNPL Putative RNA-binding protein 3 RNA-binding motif protein 3 |
| Molecular Weight(MW) | 17170 Da |
| Function | Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation (By similarity) |
| Tissue Specificity | This RBM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the Center region of human RBM3. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm (By similarity). Cell projection, dendrite (By similarity) Note=Localizes in mRNA granules in dentrites (By similarity) |
| Entrez Gene | 5935 |
|---|
Application
Western blot analysis of RBM3 antibody (Center) (Cat.# AP7397c) in NIH-3T3 cell line lysates (35ug/lane). RBM3 (arrow) was detected using the purified Pab.
Application
RBM3 Antibody (Center) (RB18776) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RBM3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RBM3 Antibody (Center) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of RBM3 (arrow) using rabbit polyclonal RBM3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM3 gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
