WB
HeLa cells were subjected to SDS PAGE followed by western blot with UBE2C antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using UBE2C Antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-UBE2C (IP:3ug; Detection:1:300) with HeLa cells lysate 2.5mg.| Product Name | UBE2C Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human UBE2C protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:200-1:1000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2C |
|---|---|
| Gene Synonyms | UBCH10 dJ447F3.2 |
| Gene Full Name | ubiquitin conjugating enzyme E2 C |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013] |
| Alternative Names | (E3-independent) E2 ubiquitin-conjugating enzyme C E2 ubiquitin-conjugating enzyme C EC:2.3.2.23 EC:2.3.2.24 UB E2C |
| Molecular Weight(MW) | 19kDa |
| Cellular Localization | cytosol,nucleoplasm,nucleus,plasma membrane,ubiquitin ligase complex. |
WB
HeLa cells were subjected to SDS PAGE followed by western blot with UBE2C antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using UBE2C Antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-UBE2C (IP:3ug; Detection:1:300) with HeLa cells lysate 2.5mg.| Application Notes | WB:1:200-1:1000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
