WB
Western blot analysis of UBE2C on different lysates with Rabbit anti-UBE2C antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HEK-293 cell lysate, Lane 3: THP-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | UBE2C Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human UBE2C aa 80-179 / 179. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | UBE2C |
|---|---|
| Gene Synonyms | UBCH10 dJ447F3.2 |
| Gene Full Name | ubiquitin conjugating enzyme E2 C |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013] |
| Alternative Names | Cyclin selective ubiquitin carrier protein antibody dJ447F3.2 antibody Mitotic specific ubiquitin conjugating enzyme antibody UB E2C antibody UBCH 10 antibody UbcH10 antibody UBE 2C antibody Ube2c antibody UBE2C_HUMAN antibody Ubiquitin carrier protein C antibody Cyclin selective ubiquitin carrier protein antibody dJ447F3.2 antibody Mitotic specific ubiquitin conjugating enzyme antibody UB E2C antibody UBCH 10 antibody UbcH10 antibody UBE 2C antibody Ube2c antibody UBE2C_HUMAN antibody Ubiquitin carrier protein C antibody Ubiquitin carrier protein E2 C antibody Ubiquitin carrier protein E2C antibody Ubiquitin conjugating enzyme E2 C antibody Ubiquitin conjugating enzyme E2C antibody Ubiquitin protein ligase C antibody Ubiquitin-conjugating enzyme E2 C antibody Ubiquitin-protein ligase C antibody |
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Cytosol, Nucleoplasm, Nucleus. |
WB
Western blot analysis of UBE2C on different lysates with Rabbit anti-UBE2C antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HEK-293 cell lysate, Lane 3: THP-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
