WB
Western blot analysis of SIRP alpha on different lysates with Rabbit anti-SIRP alpha antibody at 1/500 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: HL-60 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of THP-1 cells labeling SIRP alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | SIRP alpha Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human SIRP alpha aa 395-504 (Cytoplasmic). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:1000 IHC:1:1000 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SIRPA |
|---|---|
| Gene Synonyms | BIT MFR P84 SIRP MYD-1 SHPS1 CD172A PTPNS1 |
| Gene Full Name | signal regulatory protein alpha |
| Gene Summary | The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Signal regulatory protein alpha type 1 antibody Bit antibody Brain Ig like molecule with tyrosine based activation motifs antibody Brain Ig-like molecule with tyrosine-based activation motifs antibody Brain immunoglobulin like molecule with tyrosine based activation motifs antibody CD172 antigen like family member A antibody CD172 antigen-like family member A antibody CD172a antibody CD172a antigen antibody Inhibitory receptor SHPS-1 antibody Signal regulatory protein alpha type 1 antibody Bit antibody Brain Ig like molecule with tyrosine based activation motifs antibody Brain Ig-like molecule with tyrosine-based activation motifs antibody Brain immunoglobulin like molecule with tyrosine based activation motifs antibody CD172 antigen like family member A antibody CD172 antigen-like family member A antibody CD172a antibody CD172a antigen antibody Inhibitory receptor SHPS-1 antibody Macrophage fusion receptor antibody MFR antibody MYD 1 antibody Myd 1 antigen antibody MyD-1 antigen antibody p84 antibody Protein tyrosine phosphatase non receptor type substrate 1 antibody PTPNS1 antibody SHP substrate 1 antibody SHPS-1 antibody SHPS1 antibody SHPS1_HUMAN antibody Signal regulatory protein alpha 2 antibody Signal regulatory protein alpha 3 antibody Signal regulatory protein alpha antibody Signal regulatory protein alpha type 2 antibody Signal-regulatory protein alpha-1 antibody Signal-regulatory protein alpha-2 antibody Signal-regulatory protein alpha-3 antibody SIRP antibody Sirp-alpha-1 antibody Sirp-alpha-2 antibody Sirp-alpha-3 antibody SIRPA antibody SIRPalpha antibody SIRPalpha1 antibody SIRPalpha2 antibody SIRPalpha3 antibody Tyrosine phosphatase SHP substrate 1 antibody Tyrosine protein phosphatase non receptor type substrate 1 antibody Tyrosine-protein phosphatase non-receptor type substrate 1 antibody |
| Molecular Weight(MW) | 55kDa(Observed band size: 75-100kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of SIRP alpha on different lysates with Rabbit anti-SIRP alpha antibody at 1/500 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: HL-60 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of THP-1 cells labeling SIRP alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:1000 IHC:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
