WB
Western blot analysis of various lysates using CD172a/SIRPα Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.ICC/IF
Immunofluorescence analysis of H9C2 cells using CD172a/SIRPα Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of U2OS cells using CD172a/SIRPα Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining| Product Name | CD172a/SIRPα Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRPA |
|---|---|
| Gene Synonyms | BIT MFR P84 MYD1 SIRP MYD-1 SHPS1 CD172A PTPNS1 |
| Gene Full Name | signal regulatory protein alpha |
| Gene Summary | The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | BIT MFR P84 SIRP MYD-1 SHPS1 CD172A PTPNS1 CD172a/SIRPα |
| Molecular Weight(MW) | 55kDa(Observed MW 55kDa/120kDa) |
| Cellular Localization | Membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using CD172a/SIRPα Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of H9C2 cells using CD172a/SIRPα Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of U2OS cells using CD172a/SIRPα Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
