WB
Various lysates were subjected to SDS PAGE followed by western blot with KCC2/SLC12A5-Specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KCC2/SLC12A5-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5-Specific antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | KCC2/SLC12A5-Specific Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:20-1:200 IF-P:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.55mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC12A5 |
|---|---|
| Gene Synonyms | KCC2 DEE34 EIG14 hKCC2 EIEE34 |
| Gene Full Name | solute carrier family 12 member 5 |
| Gene Summary | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008] |
| Alternative Names | KCC2 KCC2/SLC12A5 SLC12A5 Electroneutral potassium-chloride cotransporter 2 hKCC2 |
| Molecular Weight(MW) | 126kDa(Observed Molecular Weight 130-150 kDa) |
| Cellular Localization | Cell membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with KCC2/SLC12A5-Specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KCC2/SLC12A5-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5-Specific antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC:1:20-1:200 IF-P:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
