WB
Western blot analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: mouse SP20 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KCC2/SLC12A5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody. KCC2/SLC12A5 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-KCC2/SLC12A5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody. KCC2/SLC12A5 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-KCC2/SLC12A5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody. KCC2/SLC12A5 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-KCC2/SLC12A5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Product Name | Anti-KCC2/SLC12A5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human KCC2/SLC12A5 recombinant protein (Position: A228-N833). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-2000 IHC:1:50-400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC12A5 |
|---|---|
| Gene Synonyms | KCC2 DEE34 EIG14 hKCC2 EIEE34 |
| Gene Full Name | solute carrier family 12 member 5 |
| Gene Summary | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008] |
| Alternative Names | hKCC2 K Cl cotransporter 2 KCC2 KIAA1176 Neuronal K Cl cotransporter SLC12A5 |
| Molecular Weight(MW) | 126kDa(Observed MW 126kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: mouse SP20 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KCC2/SLC12A5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody. KCC2/SLC12A5 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-KCC2/SLC12A5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody. KCC2/SLC12A5 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-KCC2/SLC12A5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody. KCC2/SLC12A5 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-KCC2/SLC12A5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Application Notes | WB:1:500-2000 IHC:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
