WB
Sample: Lane 1: Urinary bladder (Mouse) Lysate at 40ug Primary: Anti-Uricase at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution.IHC
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Uricase) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Product Name | Uricase Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from mouse Uricase: 21-120/303 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Uox |
|---|---|
| Gene Synonyms | Uri Uri2 UOX-2 |
| Gene Full Name | urate oxidase |
| Gene Summary | Enables urate oxidase activity. Involved in several processes, including purine nucleobase catabolic process; purine nucleoside catabolic process; and purine nucleoside monophosphate catabolic process. Located in mitochondrion. Is active in peroxisome. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; respiratory system; and sensory organ. Used to study hyperuricemia; kidney disease; and nephrogenic diabetes insipidus. Orthologous to human UOX (urate oxidase (pseudogene)). [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | UOX-2 Uri Uri2 urate oxidase (pseudogene) urate oxidase |
| Molecular Weight(MW) | 35kDa |
| Cellular Localization | Peroxisome. |
WB
Sample: Lane 1: Urinary bladder (Mouse) Lysate at 40ug Primary: Anti-Uricase at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution.
IHC
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Uricase) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
