WB
Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: K-562 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HDAC6 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | HDAC6 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human HDAC6 aa 1-50 / 1,215. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HDAC6 |
|---|---|
| Gene Synonyms | HD6 JM21 CPBHM KDAC6 PPP1R90 |
| Gene Full Name | histone deacetylase 6 |
| Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008] |
| Alternative Names | CPBHM antibody FLJ16239 antibody HD 6 antibody HD6 antibody HDAC 6 antibody HDAC6 antibody HDAC6_HUMAN antibody Histone deacetylase 6 (HD6) antibody Histone deacetylase 6 antibody JM 21 antibody CPBHM antibody FLJ16239 antibody HD 6 antibody HD6 antibody HDAC 6 antibody HDAC6 antibody HDAC6_HUMAN antibody Histone deacetylase 6 (HD6) antibody Histone deacetylase 6 antibody JM 21 antibody JM21 antibody KIAA0901 antibody OTTHUMP00000032398 antibody OTTHUMP00000197663 antibody PPP1R90 antibody Protein phosphatase 1 regulatory subunit 90 antibody |
| Molecular Weight(MW) | 131kDa(Observed band size: 160kDa) |
| Cellular Localization | Cell projection, Cytoplasm, Cytoskeleton, Nucleus. |
WB
Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: K-562 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HDAC6 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
