WB
All lanes use the Antibody at 1:2000 dilution for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin embedded Human renal cancer, using the Antibody at 1:150 dilution.ICC/IF
Immunofluorescent analysis using the antibody at 1:50 dilution.| Product Name | Rabbit Monoclonal to Calreticulin |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Calreticulin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:100-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CALR |
|---|---|
| Gene Synonyms | RO CRT SSA CALR1 cC1qR HEL-S-99n |
| Gene Full Name | calreticulin |
| Gene Summary | Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020] |
| Alternative Names | CALR CRT FLJ26680 RO SSA cC1Qr CRP55 CRTC HACBP Calreticulin |
| Molecular Weight(MW) | 48kDa(Observed MW: 55kDa) |
| Cellular Localization | Cytoplasm,Cytoplasmic vesicle,Endoplasmic reticulum,Extracellular matrix,Lysosome. |
WB
All lanes use the Antibody at 1:2000 dilution for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin embedded Human renal cancer, using the Antibody at 1:150 dilution.
ICC/IF
Immunofluorescent analysis using the antibody at 1:50 dilution.| Application Notes | WB:1:1000-1:2000 IHC:1:100-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
